Screening Ticks from Different Counties and Comparing Them Essay

Ticks leave be collected from areas in the five counties in the state where Lyme disease and other tick-borne infections are prevalent. Trapping sites go out be deposit in known tick habitats in the five counties to help in lodging and collecting small animals, including the target pests. Sherman live-traps baited with oatmeal and peanut-butter forget be set at the sites to ensnare the ticks. altogether captured animals exit be regularly and guardedly examined to allow the researchers to extract as many ticks as possible. The trapping of animals and ancestry of ticks from the captured animals will take about four weeks.All ticks collected will be placed in vials containing 75% ethanol and sent to parasitology laboratories. The screening process will be done through MasTag polymerase chain reception (PCR), a multiplication method, which is known for its fast and economic screening of tick-borne pathogens. PCR is excessively known for efficient and accurate scree ning of ticks for polymicrobial contagion. Before beginning the PCR procedure, the ticks will be removed from the vials, washed with saline containing phosphate buffer, and then match with Tri-reagent LS.The next step will involve nucleic acid extraction, where total ribonucleic acid will be suspended in about 30ml of H2O. The generation of complementary DNA will then follow where 20ml of the solution and 15ml of total RNA will be reacted development Superscript 11 Reverse Transcriptase as a catalyst. The MassTag PCR procedure will then be done using the tick panel, with primers added to the reactants to help detect the Borrelia species responsible for relapsing fever and the Powassan virus. All the MassTag PCR assays that will be done in the experiment will apply 3ml of cDNA. The final PCR products wi... ...tology 140.1 (2006) 143-147. Print.Koch, KR, and JG Burg. Relative Abundance and Survival of the Tick Amblyomma americanum Collected from light and Shaded Habitats. healt h check and Veterinary Entomology 20.1 (2006) 173-176. Print.Teel, Pete, HR Ketchum, DE Mock, RE Wright, and Otto Strey. The Gulf slide Tick A Review of the Life History, Ecology, Distribution, and Emergence as an Arthropod of Medical and Veterinary Importance. Journal of Medical Entomology 47.5 (2010) 707-722. Print.White, Stephan, and Donald Mock. Ixodus scapularis (Ascri Ixodidae) in Kansas A newborn Distribution Record. Journal of Medical Entomology 28.6 (1991) 872-873. Print.Williams, Christopher, William Davidson, Scott Lutz, and Roger Applegate. Health Status of Yankee Bobwhite Quail (Colinus virginiaus) in Eastern Kansas. Avian Diseases 44.1 (2000) 953-956. Print.